Phylogenetic Study of Curvularia on Sorghum from Indonesia Based on ITS rDNA Sequence

Iman Hidayat(1*), Indriati Ramadhani(2)

(1) Microbiology Division Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jln. Raya Jakarta-Bogor KM46 Cibinong 16911 West Java, Indonesia
(2) Microbiology Division Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jln. Raya Jakarta-Bogor KM46 Cibinong 16911 West Java, Indonesia
(*) Corresponding Author


Seven species of Curvularia were recorded from Indonesia, including species that pathogen to sorghum (Sorghum bicolor). The present study aims to determine four isolates of Curvularia isolated from sorghum in Indonesia using a combination of molecular phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequence and morphological examination. The results showed that the four isolates were genetically closed to C. lunata and C. dactyloctenicola. The morphological examination also insufficient to determine the identity of the four Curvularia isolates from sorghum until species level. Therefore, additional sequences from the partial fragments of the glyceraldehyde-3-phosphate dehydrogenase and the translation elongation factor 1-a genes are necessary to determine the identity of the Curvularia sequences isolated from sorghum in Indonesia until species level.


ITS rDNA, Indonesia, leaf spot, phylogenetic, sorghum

Full Text:



Edgar RC. 2004– Muscle: multiple sequence alligment with high accurancy and high throughput. Nucleic acids Res 32, 1792–1797. doi:10.1093/nar/gkh340.

Ellis MB. 1971– Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew.

Farr DF, Rossman AY. 2019– Fungal Databases, U.S. National Fungus Collections, ARS, USDA. Retrieved July 1, 2019, from

Felsenstein J. 1985– Confidence limits on phylogenetic: an approach using the bootstrap. Evolution 39, 783–791.

Hidayat I. 2017– Isolasi Spora tunggal. Jurnal Mikologi Indonesia 1, 45-46. 9In Indonesian language)

Index Fungorum. 2019– Index Fungorum. Retrieved July 1, 2019, from

Kumar S, Stecher G, Tamura K. 2016– MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870-1874.

Manamgoda DS, Rossman AY, Castlebury LA, Chukeatirote E, Hyde KD. 2015– A taxonomic and phylogenetic re-appraisal of the genus Curvularia (Pleosporaceae): human and plant pathogens. Phytotaxa 212, 175–198.

Marin-Felix Y, Groenewald JZ, Cai L, Chen Q et al. 2017– Genera of phytopathogenic fungi: GOPHY 1. Studies in Mycology 86, 99–216.

Shoemaker RA. 1959– Nomenclature of Drechslera and Bipolaris, grass parasites segregated from Helminthosporium. Canadian Journal of Botany 37, 879–887.

White TJ, Bruns T, Lee S, Taylor J. 1990– Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315–322, in MA Innis et al. (eds.), PCR Protocols: a guide to methods and applications. San Diego, Academic Press


Article Metrics

Abstract views : 231 | views : 216


  • There are currently no refbacks.

Copyright (c) 2019 Perhimpunan Mikologi Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.


Copyright © Perhimpunan Mikologi Indonesia (Mikoina), 2017. 
Powered By Open Journal Systems
e-ISSN: 2579-8766