Isolasi dan Identifikasi Jamur Makro Asal Taman Nasional Gunung Halimun Salak

Indriati Ramadhani(1*), Idris Idris(2), Masrukhin Masrukhin(3), Dian Alfian Nurcahyanto(4), Ruby Setiawan(5), AZN Ikhwani(6), RR Elfirta(7)

(1) Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
(2) Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
(3) Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
(4) Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
(5) Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
(6) Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
(7) Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
(*) Corresponding Author

Abstract


Isolasi dan identifikasi dilakukan pada jamur makro yang diperoleh dari Taman Nasional Gunung Halimun Salak. Penelitian ini dilakukan untuk memperoleh isolat murni dari jamur makro yang diperoleh dan mengetahui identitasnya. Isolat murni jamur makro diperoleh dari isolasi jaringan tubuh buah pada media PDA+ antibiotik. Identifikasi dilakukan secara morfologi dan molekuler pada isolat yang diperoleh. Sebanyak 14 isolat jamur makro anggota filum Basidiomycota dan Ascomycota telah berhasil diisolasi dari 38 spesimen jamur makro dari Taman Nasional Gunung Halimun Salak. Namun, hanya sebanyak 8 dari 14 isolat yang dapat diidentifikasi molekuler dengan baik. Kedelapan spesimen jamur makro tersebut tergolong ke dalam 2 filum, 2 kelas, 4 marga, dan 7 suku. Sebanyak 3 isolat dapat diidentifikasi molekuler dengan baik hingga tingkat spesies yakni Xylaria schweinitzii, Agaricus flocculosipes, dan Fomitopsis feei. Kelima isolat lainnya hanya dapat diidentifikasi hingga level genus yakni Ganoderma sp., Pleurotus sp., Rigidoporus sp., Gymnopus sp., dan Agaricus sp. Isolat yang diperoleh selanjutnya dapat dilakukan penapisan untuk mendapatkan isolat yang potensial dan dapat dimanfaatkan dalam bioprospeksi.

Keywords


isolasi, identifikasi, jamur makro, Taman Nasional Gunung Halimun Salak.

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References


Alexopoulos, CJ, Mims CW, Blackwell M. 1996– Introductory mycology. 4th ed. Canada: John Wiley & Sons, Inc.

Atkins FC. 1974– Guide to mushroom growing. Faber and Faber, London.

Baskaran A, Chua KH, Sabaratnam V, Ram MR, Kuppusamy UM. 2017– Pleurotus giganteus (Berk. Karun & Hyde), the giant oyster mushroom inhibits NO production in LPS/H2O2 stimulated RAW 264.7 cells via STAT 3 and COX-2 pathways. BMC Complementary and Alternative Medicine 17:40-50.

Bindu SH, Charya MS. 2018– Influence of plant oils and metals on exopolysaccharide production by Fomitopsis feei. PSM Microbiology 3(3):93-97.

Callac P, Chen J. 2018– Tropical species of Agaricus. Updates on tropical mushrooms. Basic and applied research. San Cristobal de Las Casas, Chiapas, 25-38.

Catalogue of Life. 2019– Catalogue of Life, Retrieved December 1, 2019, from https://www.gbif.org/dataset/7ddf754f-d193-4cc9-b351-99906754a03b.

Dai YC, Yang ZL, Cui BK, Yu CJ, Zhou LW. 2009– Species diversity and utilization of medicinal mushrooms and fungi in China (Review). Int J Med Mushrooms 11:287–302.

Ferraz A, Parra C, Freer J, Baeza J, Âguez JR. 2000– Characterization of white zones produced on Pinus radiata wood chips by Ganoderma australe and Ceriporiopsis subvermispora. World Journal of Microbiology & Biotechnology 16: 641-645.

Gomes-Silva et al. 2014– Two new species of Rigidoporus (Agaricomycetes) from Brazil and new records from the Brazilian Amazonia. Phytotaxa 156(4): 191-200.

Halimun Salak. 2019– Taman Nasional Gunung Halimun Salak, Retrieved December 1, 2019, from https://halimunsalak.org/.

Han ML, Cui BK. 2015– Morphological characters and molecular data reveal a new species of Fomitopsis (Polyporales) from southern China. Mycoscience 56: 168-176.

Hiraishi A, Kamagata Y, Nakamura N. 1995– Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens. Journals of Fermentation Bioengineering 79: 523--529.

Hyde et al. 2019– The amazing potential of fungi: 50 ways we can exploit fungi industrially. Fungal Diversity 97: 1-36.

Karunarathna SC, Chen J, Mortimer PE, Xu JC, Zhao RL, Callac P, Hyde KD. 2016– Mycosphere Essay 8: A review of the genus Agaricus in tropical and humid subtropical regions of Asia.

Klomklung N, Karunarathna SC, Chukeatirote E, Hyde KD. 2012– Domestication of a wild strain of Pleurotus giganteus. Sydowia 60:39–53.

Linh, Doan Thi Phuong, et al. 2014– Cytotoxic constituents from the Vietnamese fungus Xylaria schweinitzii. Natural product communications 9(5):659-660.

Martinez, AT, Barrasa JM, Prieto A, Blanco MN. 1991– Fatty acid composition and taxonomic status of Ganoderma australe from southern Chile. Mycological Research 95(7):782-784.

Mendonca RT, Jara JF, Gonzalez V, Elissetche JP, Freer J. 2008– Evaluation of the white-rot fungi Ganoderma australe and Ceriporiopsis subvermispora in biotechnological applications. J Ind Microbiol Biotechnol 35:1323–1330.

Nidadavolu SHB, Gudikandula K, Pabba SK, Maringanti SC. 2013– Decolorization of triphenyl methane dyes by Fomitopsis feei. Natural Science 5(6): 30.

O`Donnell, K. 1993. Fusarium and its near relatives. In: Reynolds, D.R. & Taylor, J.W. (eds). The fungal holomorph: Mitotic, meiotic, and pleomorphic specification in fungal systematics. CAB International, Wallingford, pp. 225--233.

Osono T, Hiroshi T. 2006– Fungal decomposition of Abies needle and Betula leaf litter. Mycologia 98(2):172-279.

Petrini LE, Petrini O. 1985– Xylariaceous fungi as endophytes. Sydowia 38:216–234.

Phan CW, Lee GS, Macreadie IG, Malek SNA, Pamela D, Sabaratnam V. 2013– Lipid constituents of the edible mushroom, Pleurotus giganteus demonstrate anti-candida activity. Nat Prod Commun 8:1763–1765

Phan CW, David P, Tan YS, Naidu M, Wong KH, Kuppusamy UR, Sabaratnam V. 2014– Intrastrain comparison of the chemical composition and antioxidant activity of an edible mushroom. Pleurotus giganteus, and its potent neuritogenic properties. Sci World J. https://doi.org/10.1155/2014/378651.

Rogers JD, Ju YM, Lehmann J. 2005– Some Xylaria species on termite nests. Mycologia 97:914–923.

Ryvarden L, Johansen I. 1980– A Preliminary polyflora flora of east Africa, Fungiflora, Oslo.

Sanger F, Nicklen S, Coulson AR. 1977– DNA sequencing with chainterminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467.

Tarafder E, Dutta AK, Sarkar J, Acharya K. 2018– A new species of Agaricus sect. Brunneopicti from Eastern India. Phytotaxa 374(2):139-146.

Wang Y, Gao YN, Wang Y, Li Y, Tang LH, Bai R, Bao DP. 2016– Bioinformatic analysis of a putative immnomodulatory protein, c13717, from Ganoderma australe. Acta Edulis Fungi 23:25–29.

Widjaja, EA, Y. Rahayuningsih, R. Ubaidillah, I. Maryanto, dan JS Rahajoe. (Eds). 2014– Kekinian keanekaragaman hayati Indonesia: 1st Edition, Jakarta: LIPI Press.

Wilson AW, Desjardin DE, Horak E. 2004– Agaricales of Indonesia. 5. The genus Gymnopus from Java and Bali. Sydowia Horn 56:137-210.

White TJ, Bruns TD, Lee SB, Taylor JW. 1990– Amplification and direct sequencing of fungal RNA genes for phylogenetics. In: Innis, MA, Gelfand DH, Sninsky JJ, White TJ. (eds). PCR protocols. Academic, San Diego, pp. 315--322.

Wu et al. 2019– Resource diversity of Chinese macrofungi: edible, medicinal, and poisonous species. Fungal Diversity 98:1–76.

Zhao RL et al. 2012– Agaricus flocculosipes sp. nov., a new potentially cultivatable species from the palaeotropics. Mycoscience 53:300–311.

Zhao RL et al. 2017– A six-gene phylogenetic overview of Basidiomycota and allied phyla with estimated divergence times of higher taxa and a phyloproteomics perspective. Fungal Diversity 84(1):43–74.




DOI: http://doi.org/10.46638/jmi.v3i2.63

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